Particle transmission in a room is very much governed by the airflow within the room. This presents a study on the effect of laminar flow on particle transmission in an air condition room. The measured supply air velocity was taken using Davis Anemometer. While, the transmitted particulate matter of diameter ≥ 0.3 µm was using Particle Counter GT-521. Both of the measured values were used as an input in simulating particle transmission using COMSOL Multiphysics software. The simulation process comprise of constructing geometry space of the room, selecting physics module, assigning values and defining boundary, meshing and finally executing and computing the simulation. The results from simulation indicated that the air velocities at the occupant's area are 22.5 % to 43 %  below than ASHRAE standard but the airflow distribution at working area meet with the standard. The simulation results are validated with the measured value and found that the percentage differences between the simulated and measured values are within the range of 0.4 % to 8.45 % which is in the tolerable range of 10 %.