The removal of Escherichia coli (E. coli) via low-frequency of electromagnetic field (LF-EMF) has a potential and become a focus of interest science many years ago. LF-EMF has been known as a higher magnetic susceptibility method, which could be effective to E. coli growth without a usage of chemicals. This study aimed to consider the removal of E. coli by using LF-EMF in water abstraction for the riverbank filtration (RBF). The effect of LF-EMF with the intensity of 2 to 10mT and 50Hz on coiled column of 1mm copper wire at 1 to 6 hours was assessed. The removal of E. coli after exposing to LF-EMF on the column model was measured using most probable number (MPN/100mL) and colonies forming unit (CFU/100mL) methods. Water samples were flowed into the column at the varied period of time up to 6 hours and flowrate of 100mL/min. From the result, 100% of E. coli has been removed at 8mT after 6 hours’ exposure and contrast to 10 mT magnetic field, 100% of E. coli are removed after 4 hours’ exposure. This result is similar to the previous study, where these findings indicate that the utilization of LF-EMF is efficient in E. coli removal from RBF system. In conclusion, it was demonstrated that the intensities of LF-EMF effect to E. coli removal with the time of exposure.