Recently printed electronic field is significantly growth. Printed electronic is to develop electrical devices by printing method. Coventional printing method that have been studied for this kind of printed electronic such as flexographic, micro contact printing, screen printing, gravure and ink jet. In flexographic and microcontact printing, a printing plate is used to transfer the designed and desired pattern to substrate through conformed contact. Therefore printing plate is play a big role in this area. Printing plate making by photopolymer which used in flexographic have limitation in achieving a micro-scala of pattern size. However, printing plate of microcontact printing have an advantages in producing micro, even nano-scala size by PDMS(Polydimethylsiloxane). Hence, rapid prototyping 3D printer is used for developing a PDMS micro-scala printing plate which will be used in reel to reel (R2R) flexographic due to high speed, low cost, mass production of this type of printing process. The flexibility of 3D printer in producing any shape of pattern easily, contributed the success of this study. The finest multiple solid line array with 1mm width and 2mm gap pattern of printing plate was successfully fabricated by 3D printer master mould due to size limitation of the FDM(Fused Deposition Modeling) 3D printer nozzle itself. However, the micro-scala multiple solid line array of 100micron width and 100micron gap successfully made by nikel platting master mould. Those types of printing plate producing method is valueable since it is easy and low cost, used for micro-flexoprahic in printed electronic field.